Bacillus acidopullulyticus pullulanase for efficient debranching of amylopectin and pullulan at alpha-1,6 linkages. Helps increase glucose yield during saccharification, improve fermentability of starch adjuncts, and support high-maltose syrup and cyclodextrin workflows. Works best within recommended pH and temperature ranges. Activity 1,000–3,000 ASPU/g. Optimal pH 4.0–5.5 and 55°C–65°C. Brown liquid. MOQ 25 kg. Ships worldwide.
Pullulanase Enzyme
1,000 – 3,000 ASPU/g
Pullulanase specifically cleaves alpha-1,6 branch points in amylopectin and related substrates, enabling downstream amylases and improving conversion efficiency in starch processing, brewing, baking, and specialty carbohydrate production.
Debranches amylopectin at alpha-1,6 linkages during saccharification, boosting glucose yield by 2–4% over amylase alone.
Converts branched starch structures into better substrates for maltose formation, supporting high-maltose syrups (>50% maltose) for confectionery and food systems.
Improves fermentability of rice and corn adjuncts by debranching residual dextrins for lighter beer styles and more consistent fermentation.
Helps manage starch structure and retrogradation behavior, supporting extended bread shelf life and softer crumb texture longer.
Used alongside glucoamylase to achieve near-complete starch conversion, maximizing ethanol yield per bushel.
Provides linear glucan substrates that support CGTase conversion during cyclodextrin manufacturing.
| Enzyme Name | Pullulanase (Debranching Enzyme) (EC 3.2.1.41) |
| Activity | 1,000 – 3,000 ASPU/g |
| Optimal pH | 4.0 – 5.5 |
| Optimal Temperature | 55°C – 65°C |
| Appearance | Brown liquid |
| Source | Bacillus acidopullulyticus |
| Shelf Life | 12 months (refrigerated) |
| Packaging | 25 kg drums / 200 L drums |
| MOQ | 25 kg |
| Certifications | ISO 9001, Food Grade, Non-GMO |
Pullulanase is supplied with defined activity (ASPU/g) to support predictable debranching and improved downstream conversion in your starch or specialty carbohydrate process.
Engineered for reliable activity in pH 4.0–5.5 and 55°C–65°C, helping you maintain stable yields across batches.
ISO 9001-managed production with Food Grade and Non-GMO options, plus COA/TDS documentation for qualified procurement.
Available in 25 kg drums and 200 L drums with MOQ 25 kg, supporting stable production planning for syrup, brewing, baking, and bioethanol plants.
Pullulanase cleaves alpha-1,6 branch linkages in amylopectin and pullulan, creating more linear chains that downstream enzymes can convert more efficiently.
For best performance, use Pullulanase at pH 4.0–5.5 and 55°C–65°C.
The ASPU/g range indicates measured enzyme activity per gram. Higher activity typically enables faster debranching at the same dosage; final dosing should be confirmed in your process trials.
Yes. In starch saccharification workflows, debranching can improve access for other enzymes and is commonly associated with higher glucose yield (reported 2–4% improvement over amylase alone).
Store refrigerated to achieve a 12-month shelf life. Keep the product sealed and avoid unnecessary temperature cycling; follow the COA/TDS storage guidance for your batch.
Tell us your application and target dosage. We’ll send a free sample, COA, and competitive pricing within 24 hours.